Make a checklist in advance for all the items which you would be carrying during the bus journey. Check this list prior to leaving your home to make sure that you have not missed any item.
In case you want to take a longer break at some bus stop due to natures call then inform the bus conductor about the delay so that he will wait for you.
Before you start the journey, always check the route and make sure its safe to travel from that route specially if you are planning to travel during night.