When your destination is about to arrive, just be ready with your bags and count all the items. Most of the people do this mistake and they often miss their belongings in the bus.
Before you start the journey, always check the route and make sure its safe to travel from that route specially if you are planning to travel during night.
Take cell number of bus conductor. In case you miss the bus at any stop then it will help you to track him or catch the bus at next stop.