When your destination is about to arrive, just be ready with your bags and count all the items. Most of the people do this mistake and they often miss their belongings in the bus.
Before you start the journey, always check the route and make sure its safe to travel from that route specially if you are planning to travel during night.
While getting down from bus at an intermediate bus stop, always ask the conductor about the break time and come back at your seat just two to three minutes before.